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KMID : 0384119900100020369
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Korean Journal of Clinical Pathology
1990 Volume.10 No. 2 p.369 ~ p.376
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Comparison of Indirect Immuofluorescence and Iodine Stainings in Detection of Chlamydia Trachomatis by Cell Culture
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K. S. Jeon
T. Y. Choi/C. W. Kim
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Abstract
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Some of sexually transmitted urogenital infection, epididymitis, suppurative vaginitis, endometritis, rectitis, and blindness as well as conjunctivitis in neonate and adult are caused by Chlamydia trachomatis. But, in clinically it was very difficult to isolate its causative ageuts. Recently, Chlamydia are isolated in McCoy cell culture and detected by iodine and staining indirect immunofluorescence methods. 89 clinical specimens from Chlamydial infection patients and 20 control strains (ATCC, D/uw-31cx) were studied for comparison of above two staining methods. Standard strains were diluted to 1: 100, 1: 10,000, 1: 100,000 and inoculated into cell culture, respectively. The results obstained were summerized as follows. 1. Positive cases in 89 clinical specimens by iodine and IIF staining were 6 (6.7 % ) and 10 (11% ) respectively. 2. Number of inclusion bodies in IIF staining were observed much more than iodine staining in positive samples. 3. There was no different sensitivity observed between iodine and IIF staining in 10 dilution of control strains( P >0.05). 4. There was markedly different sensitivity betwen iodine and IIF staining in 10.000 and 100,000 dilutions of control strains( P < 0.05, and P < 0.01) . 5. Number of inclusion bodies in IIF staining were observed more than iodine staining in 10,000 and much more in 100,000 dilution. In conclusion, it is recommended that IIF staining is more preferable than iodine staing in McCoy cell culture for detection of C. trachomatis particularly when fewer against were cultivated in the cell culture.
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